Petutschnig,b lothar altschmied,c doreen meichsner,a irena sherameti,a julian dindas,d anna mrozinska,a christian paetz,e sandra s. Production of a potential liquid plant biostimulant by. Piriformospora indica in vitro raised leguminous plants. The symbiotic interaction promotes plant performance, growth and resistancetolerance against abiotic and biotic stress. Piriformospora indica promotes early flowering in arabidopsis. The microsclerotia produced by the pathogen survive in soil for more than 15 years. The tendency of the plantlet to overcome the stress in terms of revival. Piriformospora indica, a mycorrhizallike fungus able to establish associations with roots of a wide range of plants, supporting plant nutrition and increasing plant resistance and tolerance to stress, was shown to solubilise phosphate applied in the form of animal bone char habo in fermentation systems. Piriformospora is a fungal genus of the order sebacinales. While analyzing plant factors determining root colo. In this study, the effect of endophytic fungus piriformospora indica on rhizoctonia solani ag1ia, causal agent of sheath blight disease, was investigated. We demonstrate that exudated compounds from the fungus activate stress and defense responses in the arabidopsis roots and.
Due to its extensive network of hyphae, the endophyte is able to deeply explore the soil and transfer water and minerals to the plant, some of them playing an important role in mitigating the effects of drought stress. Pdf the root endophyte piriformospora indicabelongs to the hymenomycetes basidiomycota, with a relatively close relationship to rhizoctoniaand. Piriformospora indica root colonization triggers local and. Piriformospora indica has an exceptional ability to promote plant growth. Varma a, savita verma, sudha, sahay n, butehorn b, franken p. Piriformospora indica strategies for rootcolonization. Piriformospora indica confers drought tolerance on zea mays l. Piriformospora indica, discovered in the indian thar desert in 1997 varma et al. Kogel institute of phytopathology and applied zoology, research centre for biosystems, land use and nutrition, justusliebiguniversitat gie.
Piriformospora indica, a cultivable plantgrowthpromoting root endophyte article pdf available in applied and environmental microbiology 656. Cultivable endophytic mycobiont piriformospora indica in. In a genetic screen for plants, which do not show a p. Oryza sativa plants wt and avp were inoculated with p. It is a monotypic genus, containing the single endophytic rootcolonising species piriformospora indica, discovered from orchid plants in the thar desert in rajasthan, india by prof. Research paper stress promotes arabidopsis piriformospora indica interaction khabat vahabia, sedigheh karimi dorchehb, shamci monajembashic, martin westermannd, michael reichelte, daniela falkenberga, peter hemmerichc, irena sherameti a, and ralf oelmuller ainstitute of general botany and plant physiology, friedrichschilleruniversity jena, jena, germany. Piriformospora indica reduces fusarium head blight disease. The plant strengthening root endophyte piriformospora. Pdf piriformospora indica is a mutualistic rootcolonising basidiomycete that tranfers various benefits to colonized host plants including growth. Piriformospora indica sebacinales and their biotechnological. Endophytic life strategies decoded by genome and transcriptome analyses of the mutualistic root symbiont piriformospora indica. In the current study, wildtype and transgenic tobacco constitutively overexpressing arabidopsis aquaporins pip 1. Wheat biochemical response to cadmium toxicity under.
Piriformospora indica is a mutualistic rootcolonising basidiomycete that tranfers various benefits to colonized host plants including growth promotion, yield increases as well as abiotic and biotic stress tolerance. Inoculation with piriformospora indica is more efficient. During the symbiotic interaction, it has also been observed that p. Verticillium dahliae vd is a soilborne vascular pathogen which causes severe wilt symptoms in a wide range of plants. This copy of the genome of piriformospora indica was obtained from alga zuccaro at the mpi fur terrestrische mikrobiologie in order to allow comparative analyses with other fungal genomes sequenced by the joint genome institute, a copy of this genome is incorporated into mycocosm. The tendency of the plantlet to overcome the stress in terms of revival capacity was maximal in. Here we demonstrate that an exudate preparation induces cytoplasmic calcium elevation in arabidopsis roots, and the disease development requires the ethyleneactivated. The process of p solubilisation was caused most likely by proton extrusion and medium ph. Piriformospora indica has an exceptional ability to promote plant growth, protection and stress tolerance. The authors demonstrate that sebacinales are not only extremely versatile in their associations with roots, but are also almost universally present as symptomless endophytes. Wrky6 restricts piriformospora indicastimulated and. Inoculation with piriformospora indica is more efficient in. Dock, glomus fasciculatum, phosphate transporter, piriformospora indica, p uptake, 3d model.
It is proposed that phytohormones produced by the fungal symbiont play a crucial role in the interaction with the plant roots. The fungus colonizes the roots of a wide range of vascular plants, increasing their growth. Endophytics fungus piriformospora indica and its mechanism of. The root endophytic fungus piriformospora indica requires. Endophytics fungus piriformospora indica and its mechanism. In the present work we aimed at studying fungal development and host reactions in the mutualistic symbiosis of the fungal root endophyte piriformospora indica and barley 9, 10. Thus,dicaconstitutesamodel system for systemic disease resistance in cereals. It colonises the roots of a wide range of host plants. Pdf phytohormones in plant root piriformospora indica mutualism. A symbiotic association between rice and mycorrhizal fungi could effectively protect the plant against sudden or frequent episodes of drought. The basidiomycete is a model organism for species of the recently described order sebacinales, fungi that are involved in a uniquely wide spectrum of mutualistic. Research was carried out to investigate the ability of p.
It helps plant to acquire more nutrients from soil even under extreme physical and nutrient stress conditions 11 12. Piriformospora indica, a cultivable plantgrowthpromoting. This thesis reports the effect of piriformospora indica on fusarium diseases of wheat, both head blight and crown rot, with the purpose of developing a solution to control crop diseases by using natural microorganisms. In the present work, the recently discovered root endophyticfungi piriformospora indica has been optimized and tested for its. May 29, 2012 piriformospora indica, a member of the newly created order sebacinales, is extremely versatile in its mycorrhizal associations and its ability to promote plant growth. Piriformospora indica is an endophytic rootcolonizing fungal species classified in the genus piriformospora of the order sebacinales. Piriformospora indicaa mutualistic basidiomycete with an. The endomycorrhizal fungus piriformospora indica is well known for inducing disease resistance, elevation of salt tolerance and increasing biomass in symbiotic plants. Owing to its exceptional ability to efficiently promote plant growth, protection and stress tolerance, a mycorrhiza like endophytic agaricomycetes fungus piriformospora indica has received a great attention over the last few decades. Novel cultivable mycobiont piriformospora indica as plant. Piriformospora indica is a root endophyte belonging to the sebacinaceae sebacinales, basidiomycota. The fugus piriformospora indica was discovered in the thar desert. It drastically improves plant growth and overall biomass and can be easily cultivated on a variety of synthetic media. Mar 22, 2016 piriformospora indicastrategies for rootcolonization.
Uvadare digital academic repository the oxi1 kinase. Piriformospora indica, an endophytic fungus of sebacinales, colonizes the roots of many plant species including arabidopsis thaliana. A polya ribonuclease controls the cellotriosebased. Piriformospora indica, a member of the newly created order sebacinales, is extremely versatile in its mycorrhizal associations and its ability to promote plant growth. Piriformospora indica and trichoderma show mutualistic association with many plant species. Hubei collaborative innovation center for grain industryresearch center of crop stresses resistance technologies, yangtze university. Biological control of fusarium diseases of wheat by. A polya ribonuclease controls the cellotriosebased interaction between piriformospora indica and its host arabidopsis1open joy m. Piriformospora indica a mutualistic basidiomycete with an exceptionally large plant host range. Inoculation with the endophyte piriformospora indica. Piriformospora indica mycorrhization increases grain yield by. Dolatabadi hk, goltapeh em, jaimand k, rohani n, varma a. The mutualistic fungus piriformospora indica colonizes.
Piriformospora indica is a novel plant growth promoting root endophyte. Their biotechnological applications are expected to improve the quality of crops while maintaining ecologically and economically sustainable production systems. Piriformospora indica is a basidiomycete of the order sebacinales, representing a model for the study of mutualistic symbiosis and, beyond that, the plant immune system. Piriformospora indica cell wall extract as the best. Piriformospora indica protects barley from root rot caused by. Pdf piriformospora indica, a cultivable plantgrowth. Piriformospora indica is a basidiomycete fungus colonizing roots of a wide range of higher plants, including crop plants and the model plant arabidopsis thaliana. Effect of piriformospora indica on antioxidant enzymes. A new dimension in establishment and phytopromotion archana singh 1, anjana singh 2 and aj it varma 2 i department of biological sciences, michigan technological university, 1400 townsend drive, houghton, michigan 49931, usa. Piriformospora indica hymenomycetes, basidiomycota is a newly described cultivable endophyte that colonizes roots.
Effects of piriformospora indica and sebacina vermifera on growth and yield of essential oil in fennel foeniculum vulgare under greenhouse conditions. Previous studies have shown that the endophytic fungus piriformospora indica promotes the growth and early flowering in coleus forskohlii a medicinal plant and arabidopsis. Research article open access the beneficial fungus. Despite its potential use in agriculture, little is known on the molecular basis of this. With this extraordinary diversity, sebacinales with the key fungus piriformospora indica might possess remarkable significance in natural ecosystems.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Despite its potential use in agriculture, little is known on the molecular basis of this beneficial plantfungal interaction. The subcellular localization of tubbylike proteins and. Indole derivative production by the root endophyte.
Research article open access wrky6 restricts piriformospora indica stimulated and phosphateinduced root development in arabidopsis madhunita bakshi1, khabat vahabi1, samik bhattacharya2, irena sherameti1, ajit varma3, kaiwun yeh4, ian baldwin2, atul. Piriformospora indica promotes growth, seed yield and quality. Piriformospora indica sebacinaceae is a cultivable root endophytic fungus. A cell wall extract cwe from the fungus promotes the growth of wild. Root colonization by the basidiomycete fungus piriformospora indica induces host plant tolerance against abiotic and biotic stress, and. Piriformospora indica and macronutrients for plants. In many settings colonisation promotes host growth, increases yield and protects the host from fungal diseases. Piriformospora indica is a fungal endophyte, often called an arbuscular mycorrhizallike fungus, that has been shown to provide benefits to plant symbionts by increasing nutrient uptake, biomass production, flower number, and disease resistance in a wide range of plant hosts. Piriformospora indica, alternatively referred to as am like fungi1, play a significant role in the plant growth and development24. The endophytes are known to produce a diverse group of secondary metabolites, extracellular enzymes, antibiotics, antifungal, anticancer agents that can be used as therapeutic drugs, agrochemicals or. Introduction piriformospora indica, the mycorrhizae amlike fungus, has been extensively studied to be cultivable in axenic cultures, where it forms chlamydospores asexually 1. Regenerated plantlets of tobacco subjected to two different biological hardening techniques showed 8894% survival when inoculated with p.
Plant nitrogen use efficiency may be improved through symbiosis with piriformospora indica. The beneficial fungus piriformospora indica protects. This colonization phase lasts until 3 d after inoculation dai and is followed by a cell deathdependent colonization phase more than 3 dai. Pdf piriformospora indicaa mutualistic basidiomycete. In cocultivation experiments in pots for 110 days, plants bolting and flowering were observed 8 days and 9 days earlier as compared to the controls, respectively fig. The endophytic fungus piriformospora indica reprograms. The fungus has typical pearshaped chlamydospores, and thus, named as p. The root endophytic fungus piriformospora indica has shown to improve plant growth in diverse plant species under biotic stress, while limited reports have discussed the interaction of p. Colonization of distal roots and systemic root responses. Pdf piriformospora indica hymenomycetes, basidiomycota is a newly described cultivable endophyte that colonizes roots. The fungus has typical pearshaped chlamydospores, and thus, named.
Ajit verma and group, school of life sciences, jawaharlal nehru university, new delhi. Jun 29, 1998 piriformospora indica hymenomycetes, basidiomycota is a newly described cultivable endophyte that colonizes roots. If the inline pdf is not rendering correctly, you can download the pdf file here. Endophytic fungus piriformospora indica induced systemic. Effect of endophytic fungus, piriformospora indica, on. Effects of the endophyte piriformospora indica on growth, physiology and water relations of tobacco nicotiana tobacum author creator ferster, frances g. To investigate systemic effects within the root system, we established a hydroponic splitroot. Piriformospora indica as a mycofertilizer has produced a greater passion among mycorrhizologists, due to being easily culturable, plantgrowth enhancement and induction. Furch,a volker lipka,b rainer hedrich,d bernd schneider,e ales. Some facts related with the lifestyle and the mechanisms underlying root colonization of p. Coculture of arbuscular mycorrhizalike fungi piriformospora indica and sebacina vermifera with plant cells of linum album for enhanced production of podophyllotoxins.
Flowering in plants is synchronized by both environmental cues and internal regulatory factors. Mar 22, 2016 owing to its exceptional ability to efficiently promote plant growth, protection and stress tolerance, a mycorrhiza like endophytic agaricomycetes fungus piriformospora indica has received a great attention over the last few decades. Piriformospora indica is an endophytic fungus that colonized monocot as well as dicot. Piriformospora indica, as a plant growthpromoting mycorrizal fungus pgpf, has the ability to colonize the root cortex of a broad variety of monocot and dicot plant species 5, 6.
In our recent study, we have characterized a high affinity phosphate transporter pipt and by using rnai approach. In addition, plant defence responses activated in p. Inoculation with the fungus and application of fungal culture filtrate promotes plant growth and biomass production. The beneficial fungus piriformospora indica protects arabidopsis from verticillium dahliae infection by downregulation plant defense responses chao sun1, yongqi shao2, khabat vahabi1, jing lu2,6, samik bhattacharya2, sheqin dong3, kaiwun yeh4, irena sherameti1, binggan lou5, ian t baldwin2 and ralf oelmuller1 abstract.
Piriformospora indica and plants dissertation zur erlangung des doktorgrades dr. Cell wall extract from piriformospora indica enhances. Here we demonstrate that an exudate preparation induces cytoplasmic calcium elevation in arabidopsis roots, and the disease development requires the ethyleneactivated transcription factor. Piriformospora indica promotes growth, seed yield and.
Piriformospora indica is an endophytic fungus that colonizes roots of many plant species and promotes growth and resistance to certain plant pathogens. The fungus is characterized by a broad host spectrum encompassing various monocots and. The interaction of arabidopsis with piriformospora indica. Phytohormones in plant rootpiriformospora indica mutualism. The aim of the present study is to evaluate the effect of p. The root endophyte piriformospora indicabelongs to the hymenomycetes basidiomycota, with a relatively close relationship to rhizoctoniaand sebacina. Piriformospora indica protects barley from root rot caused by fusarium graminearum. Beneficial effects elicited by the root endophyte piriformospora indica are widely known, but the mechanism by which these are achieved is still unclear. Piriformospora indica stimulates root metabolism of. Among pgpm, the root endophytic fungus piriformospora indica. Piriformospora indica basidiomycota, sebacinales is a rootendophytic fungus which is able to improve growth and overall biomass of both monocot and eudicot plants and increase tolerance to biotic and abiotic stresses.
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